Introduction to the Luminescent Bacteria Method来源： 作者: 阅读：126657 网友评论0条 Updated: 2011-02-23 14:28 Source: Author: reading: 126 657 2010 0 comments
In recent years, water pollution has become increasingly serious. At the same time, many sensitive and effective environmental monitoring methods have been developed. These methods can be divided into two categories: analytical technology and biological monitoring. Among them, the analysis technology is often used to test the conventional indicators of wastewater, but it cannot reflect the comprehensive toxicity of water quality. Traditional biological monitoring uses daphnia, algae or fish as test subjects. Although it can reflect the direct impact of poisons on living things, the biggest disadvantage of these methods is that the experimental period is long and the experimental process is tedious. Aiming at the deficiency of traditional biological toxicity detection methods, research and development of a new biological toxicity monitoring technology-luminescent bacteria method. The method is easy to operate and the measurement results are clear at a glance. It is favored by scientific research units and enterprises.
Since 1672 After Boyle observed that the light emitted by luminescent bacteria was easily suppressed by chemicals, many scientists have conducted a lot of research on the luminescent effect of bacteria. From the 1970s to the early 1980s, foreign scientists for the first time isolated and screened luminescent bacteria from the surface of marine fish that were harmless to the human body and sensitive to the environment. They were used to detect biological toxicity in water bodies. testing method. This technology was introduced in China in the early 1980s, and seawater and freshwater (Vibrio qinghaiensis) light-emitting bacteria have been isolated to detect acute biological toxicity of environmental pollutants.
Generally, luminescent bacteria are about 1.5-3um (micrometers) long and 0.5-0.8um in width, so they can't be seen by the naked eye. They can only be distinguished when they are magnified to 1000 times with a microscope. And their luminescence can only be seen under certain conditions. Vibrio Qinghai is currently the only non-pathogenic freshwater photobacterium, so the patented Vibrio qinghai freeze-dried powder is safe and reliable during transportation and use. It does not require special treatment of the discarded bacterial solution and will not cause secondary pollution. .
|Glowing bacteria in a petri dish|
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